The nitrite anion is an endogenous product of mammalian nitric oxide (NO) metabolism, a key intermediate in the nitrogen cycle in plants, and a constituent of many foods. Intracarotid (i.c.) Nitric oxide inhibited O2+ produced by xanthine oxidase only when added simultaneously with substrate, consistent with the short half-life of NO in oxygenated solution. These data support the view that nitric oxide plays a significant role in modulating basal vasomotion and endothelial-dependent dilation stimulated by acetylcholine or increase in blood flow in epicardial coronary arteries and also influence the regulation of coronary blood flow during physiologic conditions. Animal models that show vascular dysfunction demonstrate the protective role of endothelial NO dependent pathways. This study examined the contribution of nitric oxide (NO) to the susceptibility or resistance to the hypertensive effects of high sodium chloride (8.0% NaCl) intake in young Dahl/Rapp salt-sensitive (SS/Jr) and salt-resistant (SR/Jr) rats. Elevated levels of ADMA have been reported in many conditions associated with a high cardiovascular risk. Conclusions. In several segments each of four normal coronary arteries, acetylcholine caused a dose-dependent dilation from a control diameter of 1.94 +/- 0.16 mm to 2.16 +/- 0.15 mm with the maximal acetylcholine dose (P less than 0.01). Thus, impaired endothelium-dependent relaxation in atherosclerosis is neither due to decreased activity of the enzyme responsible for the production of nitrogen oxides from arginine nor to arginine deficiency. Since nitrite has been shown to be potently cytoprotective and restore NO biochemical homeostasis, we investigated if supplemental nitrite could attenuate microvascular inflammation caused by a high cholesterol diet. Because the production of nitrogen oxides increased in response to acetylcholine in both hypercholesterolemic and atherosclerotic vessels, impairments in signal transduction are not responsible for abnormal endothelium-dependent relaxations. Incubation of isolated cat neutrophils with L-NMMA, but not L-NAME, resulted in direct upregulation of CD11/CD18 as assessed by flow cytometry. Acetylcholine is believed to dilate normal blood vessels by promoting the release of a vasorelaxant substance from the endothelium (endothelium-derived relaxing factor). Mean urinary cGMP levels were higher in salt-sensitive rats on oral L-arginine than salt-sensitive rats on D-arginine. Administration of nitrite in the drinking water inhibited the leukocyte adhesion and emigration and prevented the arteriolar dysfunction. A subset of hearts from each group (n = 8 for control and n = 7 for GK groups) were freeze-clamped for determining baseline values after the initial perfusion of 24 min. to assess the possibility of reversing endothelial dysfunction by enhancing the release of nitric oxide from the endothelium. 1998; 95: 939–950. The endothelial nitric oxide synthase (eNOS) generates the vasoprotective molecule nitric oxide (NO), which plays a central role in the control of vascular hemostasis. Sessa WC, Pritchard K, Seyedi N, Wang J, Hintze TH (1994) Chronic exercise in dogs increases coronary vascular nitric oxide production and endothelial cell nitric oxide synthase gene expression. Key risk factors for atherosclerosis, including hypertension, dyslipidemia, obesity, and diabetes, can be linked specifically to abnormalities in nitric oxide (NO)–mediated endothelial vasodilation.6, 7, 8, 9 Earlier, related hypotheses of atherogenesis focused on the exacerbation of proinflammatory processes in response to these conditions (e.g., both hypertension and dyslipidemia … Released by the inner layer of cells (endothelium) of blood vessels, nitric oxide relaxes the vessels and keeps them flexible, allowing them to dilate, boosting blood flow, and helping to … Our current findings uncover a novel mechanism by which AMPK protects against hypercholesterolemia-mediated endothelial dysfunction. ESMIRO mice had preserved glucose homeostasis and were normotensive. Lipofundin MCT/LCT Inhibits Levcromakalim-Induced Vasodilation by Inhibiting Endothelial Nitric Oxide Release, Urinary Dimethylamine (DMA) and Its Precursor Asymmetric Dimethylarginine (ADMA) in Clinical Medicine, in the Context of Nitric Oxide (NO) and Beyond, Effet des précurseurs de monoxyde d'azote sur la fonction vasculaire et la performance à l'exercice, Recent Advances in Manufacturing Innovative Stents, Immunotoxic mechanisms of cigarette smoke and heat-not-burn tobacco vapor on Jurkat T cell functions *. All the patients with variant angina had angina, electrocardiographic changes, and localized occlusive epicardial coronary-artery spasm at concentrations of 10(-6) (n = 2) or 10(-5) (n = 3) mol per liter. The consequent generation of NO modulates platelet reactivity by increasing cyclic GMP. 5 With age, the endothelium gradually loses its responsiveness, which leads to a reduction of the amount of blood that flows through the body. Small arteries were dissected from gluteal subcutaneous biopsies and mounted on a wire-myograph. The purpose of this study was to determine whether infusion of L-arginine, the precursor of the endothelium-derived relaxing factor, nitric oxide, improves endothelium-dependent vasodilation in hypercholesterolemic humans. Bradykinin caused concentration-dependent release of NO from the cells in amounts sufficient to account for the biological activity of EDRF. g wet wt(-1)) ischemia. Long-term treatment has also received considerable attention (e.g. Selective endothelial insulin resistance is sufficient to induce a reduction in NO bioavailability and endothelial dysfunction that is secondary to increased generation of reactive oxygen species. Received antihypertensive drugs for more than 15-fold ACh ) and after reperfusion ( P < 0.05 ) impairs... As other constitutive nitric-oxide synthases inflammation and endothelial function, in part, to endothelial dysfunction in pathogenesis... 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